2015 Fiscal Year Final Research Report
Study on mechanism of placental toxicity of folic acid antagonist, methotrexate
| Project/Area Number |
25450423
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 胎盤毒性 |
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| Outline of Final Research Achievements |
We clarified that Methotrexate (MTX) exposure during gestation day (GD) 11-12 induced hypoplasia of placental basal cell layer in rats. In the present study, the histopathological and molecular biological examinations were performed to clarify the pathogenesis of MTX-induced hypoplasia of placental basal cell layer. The suggestions of the present study were as follows: (1) Apoptosis in hypoplasia of placental basal cell layer was induced by ① both extrinsic and intrinsic pathways, ② oxidative damage of protein and unsaturated fatty acid, and ③ activation of JNK(c-Jun N-terminal kinase)pathway, (2) There were no relation between sensibility to MTX of component cells in placenta and expression levels of dihydrofolate reductase.
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| Free Research Field |
毒性病理学
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