Study of oral vaccine prevent from neosporosis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450432
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: Ike Kazunori 日本獣医生命科学大学, 獣医学部, 教授 (50159597)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Neospora caninum / ネオスポラ症 / ワクチン / 経口投与 / アジュバント / 腸管免疫

Outline of Final Research Achievements

The vaccine for neosporosis was developed using alga Chlamydomonas reinhardtii expressed with Neospora caninum surface antigens. C. reinhardtii cells were co-expressed with both Escherichia coli heat-labile enterotoxin B subunit (LT-B) and N. caninum surface antigen, NcSAG1 or NcBAG1. The C. reinhardtii suspensions were inactivated with heating 56℃ for 15 min, and prepared on concentration 5×10＾6/mL as the vaccines. The vaccines were orally inoculated by needle with 0.5 mL/head twice a week. The mice were bled from a caudal vein every week, and antibodies were measured by ELISA. The antibodies were appeared from 3 week after inoculation, and so on. These results suggested that vaccine using alga C. reinhardtii was useful for antibody production by oral.

Free Research Field

獣医寄生虫学