Characterization of novel ADP-ribosyltransferase toxin from Salmonella which cause Salmonellosis in adult cattle.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450434
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: UCHIDA Ikuo 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門 細菌・寄生虫研究領域, 領域長 (70355204)
• Co-Investigator(Kenkyū-buntansha): TAMAMURA Yukino 国立研究開発法人・農業・食品産業技術総合研究機構, 動物衛生研究部門・細菌・寄生虫研究領域, 研究員 (90584384)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: サルモネラ症 / ADP-リボシル化毒素 / 病原性因子 / 百日咳毒素 / G蛋白質 / 複合体毒素

Outline of Final Research Achievements

Salmonella Typhimurium DT104 produces pertussis-like toxin (ArtAB-DT104), which catalyzes ADP-ribosylation of G proteins. Here, we characterized ArtAB-DT104 and homologs in S. Worthington and S. bongori (ArtAB-SW and ArtAB-Sb, respectively). ArtABs comprise a A subunit and pentameric B subunits. The A subunits of ArtAB-SW and ArtAB-Sb, which exhibit ADP-ribosyltransferase activity, had high amino acid sequence identity with ArtAB-DT104, whereas the B subunits of ArtAB-Sb showed lower identity. Injection of ArtABs was fatal in mice; LD50 of ArtAB-DT104 and ArtAB-SW were lower than that of ArtAB-Sb, suggesting that the B subunit is important for ArtAB toxicity. ArtABs catalyzed ADP-ribosylation of Gi proteins in RAW264.7 cells and increased intracellular cyclic AMP levels. Unlike Pertussis toxin, ArtABs did not induce lymphocytosis, possibly due to differences in ADP-ribosylation of target G proteins. These results suggest the general importance of ArtABs for Salmonella virulence.

Free Research Field

獣医学