Pathological study on pathogenicity of emerging arboviruses to cattle

2017 Fiscal Year Final Research Report

• Project/Area Number: 25450435
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Tanaka Shogo 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, ユニット長 (10355216)
• Co-Investigator(Kenkyū-buntansha): 梁瀬 徹 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, 上級研究員 (90355214)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Kumiko 国立研究開発法人農研機構, 動物衛生研究部門・病態研究領域・病理ユニット, 上級研究員 (30391444) ; SHIRAFUJI Hiroaki 国立研究開発法人農研機構, 動物衛生研究部門・越境性感染症研究領域・暖地疾病防除ユニット, 主任研究員 (40414726) ; KUBOTA Chikara 鹿児島大学, 共同獣医学部, 教授 (80420652)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Keywords: アルボウイルス / 感染実験 / 病原性 / ピートンウイルス / 牛胎子

Outline of Final Research Achievements

To clarify pathogenicity of emerging arboviruses suspected as a cause of cattle congenital abnormalities, cultured myoblast lines derived from an early, middle- and full-term gestational bovine fetus were established, and compared viral tropism on each period by measuring the arbovirus-specific gene amount after inoculation. We also inoculated with Peaton virus (PEAV) in middle-term bovine fetuses and attempted to reproduce the congenital abnormalities, and pathologically examined other bovine fetuses on the 10-day after inoculation. As a result, PEAV showed high proliferative affinity to myoblasts derived from the early fetus, suggesting high sensitivity to early bovine fetuses. In addition, although the congenital anomalies could not reproduce by experimental infection with PEAV in the middle-term bovine fetuses, pathogenicity to cattle was confirmed by the onset of non-suppurative meningoencephalomuyelitis, detection of PEAV genes and antigen localization in the affected fetuses.

Free Research Field

獣医学