2015 Fiscal Year Final Research Report
New approach of cancer research; mitochondrial respiratory chain inhibitors as a new therapeutic target.
| Project/Area Number |
25450442
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 犬乳腺癌 / ミトコンドリア呼吸鎖 / AMPK / メトホルミン / ニコチンアミドアデニンジヌクレオチド |
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| Outline of Final Research Achievements |
The multi-drug screening showed the high sensitivity of a malignant clone of canine mammary gland tumor (cMGT) cell line, CHMp-5b to mitochondrial respiratory chain inhibitors (MRCIs). While Metformin, one of the MRCIs, exhibited its anti-tumor effect through AMPK pathway on low sensitive clonal cell line, CHMp-13a, it induced G0/G1 cell cycle arrest in AMPK-independent manner on CHMp-5b. Cellular nicotinamide adenine dinucleotide (NAD) amount was decreased by metformin which exerted anti-proliferative effect, though the ATP amount was not changed. These results highlighted potential of metformin as a novel treatment for cMGT as well as a novel NAD-dependent mechanism.
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| Free Research Field |
臨床獣医学
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