2015 Fiscal Year Final Research Report
Application of INSL3 measurements for clinical application and expression analyses of INSL3 receptors in reproductive disorders in domestic animals
Project/Area Number |
25450448
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Veterinary medical science
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Research Institution | Osaka Prefecture University |
Principal Investigator |
Kawate Noritoshi 大阪府立大学, 生命環境科学研究科(系), 准教授 (80221901)
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Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI MASAHIRO 大阪府立大学, 生命環境科学研究科, 助教 (50582334)
TAMADA HIROMICHI 大阪府立大学, 生命環境科学研究科, 教授 (10155252)
INABA TOSHIO 大阪府立大学, 生命環境科学研究科, 教授 (00137241)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | INSL3 / テストステロン / RXFP2 / 精液性状不良牛 / 卵巣嚢腫牛 / ウシ胎子雌雄判別 / 潜在精巣 / 子宮蓄膿症犬 |
Outline of Final Research Achievements |
In the present study, we examined an application of INSL3 measurements in maternal peripheral blood and urine of pregnant cattle. As the results, we found that nearly 90% of accuracy for fetal gender prediction was obtained when we used data of blood testosterone and INSL3 concentrations at multiple-time points from the mid to late gestation. There were no significant differences of blood INSL3 concentrations, between bulls with abnormal and normal semen, and also between cows with ovarian cysts and normal ovarian activity. We also investigated expression of INSL3-Receptor (RXFP2) in retained testes in cryptorchid dogs, and elucidated that loss of RXFP2 gene expression in spite of substantial expression of INSL3 in retained testes. No significant differences were found in INSL3 and RXFP2 gene expressions in ovaries and uteruses between dogs with pyometra and normal dogs.
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Free Research Field |
獣医繁殖学
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