Development of oral inactivated multivalent Salmonella vaccine using E. coli cell surface display system

2016 Fiscal Year Final Research Report

• Project/Area Number: 25450449
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: Tani Hiroyuki 大阪府立大学, 生命環境科学研究科, 准教授 (00305658)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: サルモネラ / 不活化ワクチン / 大腸菌表層ディスプレイ

Outline of Final Research Achievements

In order to develop oral inactivated multivalent Salmonella vaccine, the fusion proteins of cholera toxin B subunit (CTB) or heat-labile enterotoxin B subunit (LTB) of ETEC, which are the suitable candidates for mucosal adjuvant, and fliC as Salmonella Enteritidis (SE) vaccine candidate protein were expressed on the surface of E. coli by using E. coli surface display system. The chickens were immunized by oral, intra-muscular or intra-ocular route with the inactivated E. coli expressed fusion proteins. SE flagellar specific serum IgG titer and intestinal mucous IgA concentration were increased after immunization. The immunized chickens with intra-ocular administration of inactivated E. coli expressed LTB and fliC fusion protein showed significantly decreased bacterial counts in the cecal contents. Our findings obtained in this study can be applied to development of simple and safety mucosal Salmonella vaccine.

Free Research Field

獣医学