Development for bovine protothecal vaccine

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450452
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Nihon University
• Principal Investigator: KANO Rui 日本大学, 生物資源科学部, 准教授 (00318388)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Kazuyuki 酪農学園大学, 獣医学群, 教授 (30339296)
• Research Collaborator: ITO Takaaki
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 牛プロトテカ性乳房炎 / 血清学的診断法 / 不活化抗原 / ELISA / Prototheca zoppfii

Outline of Final Research Achievements

Prototheca zopfii is an achlorophyllic alga that causes bovine mastitis, resulting in a reduction in milk production and the secretion of thin, watery milk with white flakes. This study evaluated the use of an ELISA system for distinguishing cows with mastitis due to P. zopfii genotype 2 from cows with chronic candidal mastitis and healthy cows. Moreover, we investigated the transitional changes of specific antibody titer of healthy cows injected with inactivated P. zopfii genotype-2 cells. This ELISA system exhibited the highest sensitivity (94%) and specificity (100%) for the chronic protothecal mastitis cows when the positive cutoff value was set at 43.4 EU. Anti-protothecal IgG titers were positive in all cows after inoculation with inactivated P. zopfii genotype 2 cells. These results indicated that ELISA detection of anti-prototheca IgG in serum provided specificity and sensitivity sufficient for use in diagnosing protothecal mastitis.

Free Research Field

獣医臨床病理学