Molecular analysis and development of a DNA-testing method for erythrocyte blood group antigens

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450454
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: OMI TOSHINORI 日本獣医生命科学大学, 獣医学部, 教授 (40296091)
• Co-Investigator(Kenkyū-buntansha): TUCHIDA Shuichi 日本獣医生命科学大学, 獣医学部, 教授 (20217326) ; BONKOBARA Makoto 日本獣医生命科学大学, 獣医学部, 教授 (50343611)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 輸血医療 / 遺伝子検査 / 血液型物質 / 小動物

Outline of Final Research Achievements

The dog erythrocyte antigen (DEA) and cat AB blood group system are important in transfusion-related research. Here we investigated the molecular basis of dog and cat erythrocyte blood group antigens. Of 282 dogs, 71.6% were positive and 28.4% were negative for DEA 1.1. Of 772 cats, 95.1%, 4.9%, and 0% of cats had the A, B, and AB blood group, respectively. To identify a candidate molecule expressed on the dog erythrocyte membrane associated with dog blood group, we performed the agglutination test using a lectin (anti-NeuGC). Dog RBCs were then divided into two types: positive and negative cells. Results suggested the CMAH gene associated with the NeuGC expression may be associated with the DEA system. In cat,the CMAH gene encodes the antigens in the cat AB blood group system. In the gene, we identified eight SNPs in blood type B cats, including four novel SNPs. These data provide information for developing a DNA-testing method to identify blood groups.

Free Research Field

動物生命科学