ネコ肥満の早期遺伝子診断法の開発

2015 Fiscal Year Annual Research Report

• Project/Area Number: 25450456
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: 山本 一郎 日本獣医生命科学大学, 獣医学部, 准教授 (00424763)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ネコ / GPR120 / GPR40

Outline of Annual Research Achievements

肥満に関与する遊離脂肪酸の特異的受容体（FFAR1 / GPR40およびFFAR4 / GPR120）のネコでの機能解析を行うとともに、同遺伝子に存在するSNPが肥満に関与するか否かを検討した。結果、ネコGPR40は他動物と異なる特殊な21アミノ酸残基が付加され、特異なC末端構造であることが明らかとなった他、申請者が共同研究を行った結果、肥満イヌのGPR120遺伝子中にSNPが存在することを明らかにした。同SNPは肥満犬を遺伝子診断により発見する貴重なマーカーとして有用であることが示唆された。今後、肥満ネコのSNP検出は更なる検討が必要であることが示唆された。

Research Products

• [Journal Article] Genetic variants of the unsaturated fatty acid receptor GPR120 relating to obesity in dogs. (2015)
  • Author(s): Miyabe M, Gin A, Onozawa E, Daimon M, Yamada H, Oda H, Mori A, Momota Y, Azakami D, Yamamoto I, Mochizuki M, Sako T, Tamura K, Ishioka K
  • Journal Title: J Vet Med Sci Volume: 77 Pages: 1201-1206
  • DOI: 10.1292/jvms.15-0031
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of free fatty acid receptors GPR40/FFAR1 and GPR120/FFAR4 in a domestic cat. (2015)
  • Author(s): Habara M, Tamanuki M, Ishikawa S, Takemitsu H, Mori N, Okada Y, Nakao N, Kawasumi K, Ishioka K, Arai T and Yamamoto I.
  • Journal Title: Asia J Anim Vet Adv Volume: 10 Pages: 185-190
  • DOI: 10.3923/ajava.2015.185.190
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sirtuin 1 suppresses nuclear factor κB induced transactivation and pro-inflammatory cytokine expression in cat fibroblast cells. (2015)
  • Author(s): Ishikawa S, Takemitsu H, Habara M, Mori N, Yamamoto I, Arai T.
  • Journal Title: J Vet Med Sci Volume: 77 Pages: 1681-1684
  • DOI: 10.1292/jvms.15-0245
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Changes in fatty acid composition in tissue and serum of obese cats fed a high fat diet. (2015)
  • Author(s): Fujiwara M, Mori N, Sato T, Tazaki H, Ishikawa S, Yamamoto I, Arai T.
  • Journal Title: BMC Vet res Volume: 11 Pages: 200
  • DOI: 10.1186/s12917-015-0519-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Preliminary analysis of modified low-density lipoproteins in the serum of healthy and obese dogs and cats. (2015)
  • Author(s): Mori N, Okada Y, Tsuchida N, Hatano Y, Habara M, Ishikawa S, Yamamoto I, Arai T.
  • Journal Title: Front Vet Sci Volume: 2 Pages: 1-7
  • DOI: 10.3389/fvets.2015.00034
  • Peer Reviewed / Int'l Joint Research