Differentiation mechanism of type ID myofiber as a ruminant-specific super fatigue-resistant myofiber type

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450461
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Tohoku University
• Principal Investigator: WATANABE Kouichi 東北大学, (連合)農学研究科(研究院), 助教 (80261494)
• Co-Investigator(Kenkyū-buntansha): ASO Hisashi 東北大学, 大学院農学研究科, 教授 (50241625)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 超疲労耐久性筋線維 / ウシ / ３－ヒドロキシ酪酸脱水素酵素 / カルシウムイオン関連因子 / Sirtuin / カルニチンアシル基転移酵素ー１ｂ / 機能適応 / ID型筋線維

Outline of Final Research Achievements

Ruminant-specific type ID myofibers as a super fatigue-resistant myofiber, which are distributed in anti-gravity muscles involved in posture maintenance, possess strong 3-hydroxybutyrate dehydrogenase activity. In bovine muscles, our results suggest that type ID myofibers are possible to keep higher sarcoplasmic calcium ion level for long-term muscular movement, and have higher metabolic capacity of fatty acid than other myofiber types for sufficient energy supply. Moreover, it suggests that sirtuin1 activate PGC-1alpha for increasing of mitochondria in myofiber transition of "type IIB to type IIA" as oxidative change. Successively, upregulation of sirtuin3 in mitochondria seems to lead induction of fast-to-slow transformation and appearance of type ID myofiber involved in super fatigue-resistance.

Free Research Field

機能形態学