2015 Fiscal Year Final Research Report
Studies on the mechanism of persistent infection of Bombyx mori macula-like virus in silkworm-derived cell lines.
| Project/Area Number |
25450482
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | BmMLV / マキュラウイルス / カイコ / 持続感染 / 培養細胞 / チモウイルス |
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| Outline of Final Research Achievements |
The Bombyx mori macula-like virus (BmMLV) is a member of the genus Maculavirus, family Tymoviridae, and contains a positive-sense single-stranded RNA genome. Previously, we reported that almost all B. mori-derived cell lines have already been contaminated with BmMLV via an unknown infection route. To understand the mechanism of BmMLV propagation, we identified a novel subgenomic RNA of BmMLV. Also, we determined several viral inactivation methods by using the physical conditions. Furthermore, we revealed that BmMLV did not replicate in nine mammalian-derived cell lines. RNA-seq analysis was also performed to clarify the mechanism of BmMLV persistent infection.
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| Free Research Field |
昆虫ウイルス学
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