Development of novel analyses of hydrophilic compounds utilizing coordination with metal ions

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450505
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental agriculture(including landscape science)
• Research Institution: Toyama Institute of Health
• Principal Investigator: KEMMEI TOMOKO 富山県衛生研究所, 化学部, 副主幹研究員 (60416089)
• Co-Investigator(Kenkyū-buntansha): KODAMA Shuji 東海大学, 理学部, 教授 (70360807) ; YAMAMOTO Atsushi 中部大学, 応用生物学部, 教授 (60360806)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 有機酸 / 糖アルコール / 金属イオン / HPLC-UV法

Outline of Final Research Achievements

We developed simple and sensitive reversed phase liquid chromatographic methods for the analyses of hydrophilic compounds (HCs) using on-line complexation with metal ions. When metal ions are added to the mobile phase, they coordinate with HCs and therefore may influence the retention of HCs. Moreover, the absorption bands of HCs may shift to longer wavelengths and their extinction coefficients may increase. In this study, we developed reversed-phase liquid chromatographic methods with UV detection to determine organic acids as their copper complexes and alditols as their molibdate complexes. The developed methods were used for quantitative determination of organic acids and alditols in food samples.

Free Research Field

衛生化学