Structural heterogeneity of mammalian testis ribosomes: comprehensive analysis for new mechanisms of translational control

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450514
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: NADANO Daita 名古屋大学, 生命農学研究科, 准教授 (00228074)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: リボソーム / 翻訳 / 発現制御

Outline of Final Research Achievements

Our proteomic analysis of rodent cytoplasmic ribosomes revealed their structural heterogeneity. This analysis identified a zinc finger protein, Lyar, in testicular ribosomes. Lyar was suggested to be included in the 60S large subunit, but not in polysomes, by ultracentrifugation of testicular ribosomes. Furthermore, translation was increased by Lyar in vitro, pointing out the involvement of this protein in translation. Overall, our data in this study (including unpublished ones) are expected to make a connection between ribosome heterogeneity and translational control.

Free Research Field

哺乳類の分子生体制御学