2015 Fiscal Year Final Research Report
Mechanism that telomere defect make cell sensitive to anti-microtubule drug and their application
Project/Area Number |
25450517
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | Hiroshima University |
Principal Investigator |
Ueno Masaru 広島大学, 先端物質科学研究科, 准教授 (90293597)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | テロメア / スピンドルチェックポイント / 分裂酵母 / 微小管阻害剤 / 抗がん剤 |
Outline of Final Research Achievements |
Fission yeast Pot1 binds telomere DNA, and Rqh1 is required for resolution of recombination intermediate. We have shown that the double mutant between pot1 and the helicase-dead point mutant of the RecQ helicase Rqh1 (rqh1-hd) is sensitive to microtubule drug and accumulates recombination intermediates at telomeres. In this research, we found that Spindle assembly checkpoint (SAC)-dependent prometaphase arrest occurred frequently in pot1 rqh1-hd double mutants. Our results indicate that the accumulation of recombination intermediates induces SAC-dependent prometaphase arrest.
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Free Research Field |
分子細胞生物学
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