Secretome analysis for the search for novel danger signal alarmins in ATP-stimulated macrophages

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450521
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: TAKENOUCHI Takato 国立研究開発法人農業生物資源研究所, 動物生体防御研究ユニット, 上級研究員 (20292518)
• Co-Investigator(Kenkyū-buntansha): TSUKIMOTO Mitsutoshi 東京理科大学, 薬学部薬学科, 講師 (70434040)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アラーミン / マクロファージ / P2X7受容体 / GAPDH

Outline of Final Research Achievements

Alarmins are endogeneous molecules that act as danger signals to promote inflammation or innate immune response. Since they are mainly secreted from macrophage-related cells, we performed P2X7 receptor-secretome analysis using ATP-stimulated mouse microglial cells, known as brain macrophages, to search for novel alarmins. We have identified 640 different proteins as candidate molecules that may act as alarmins. Among them, we demonstrated the unconventional secretion pathway and innate immunomodulatory functions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in microglial cells. The findings suggest that GAPDH secreted from macrophages may act as a mediator of innate immune system.

Free Research Field

農学