Analysis of a dual resistance protein complex in plant defense responses

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450523
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: 岡山県農林水産総合センター生物科学研究所
• Principal Investigator: Narusaka Mari 岡山県農林水産総合センター生物科学研究所, その他部局等, 研究員 (80376847)
• Co-Investigator(Renkei-kenkyūsha): NARUSAKA Yoshihiro 岡山県農林水産総合センター, 生物科学研究所, 専門研究員 (20335459)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 抵抗性蛋白質 / 生物間相互作用 / 病害抵抗性 / シグナル伝達 / 炭疽病菌 / 免疫沈降 / シロイヌナズナ

Outline of Final Research Achievements

We found that RPS4 and RRS1 function as a dual resistance protein system that prevents infection by three distinct pathogens (Anthracnose; Colletotrichum higginsianum, Bacterial wilt; Ralstonia solanacearum, and Bacterial speck; Pseudomonas syringae pv. tomato strain DC 3000 expressing avrRps4). We demonstrated that a pair of Arabidopsis NB-LRR-type R genes, RPS4Ws and RRS1Ws, function in two Brasscicaseae, but also in two Solanaceae. In this study, we analyzed that whether a pair of neighboring genes, RPS4 and RRS1, function cooperatively as a dual resistance protein system and then this system are tightly regulated. Our finding will contribute to a new strategy for creating pathogen-resistant vegetables and crops by a transgenic approach.

Free Research Field

植物生理学