In situ monitoring of drug action using electrochemical sensors

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460036
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Yasuda Women's University (2014-2015); Okayama University (2013)
• Principal Investigator: KATSU Takashi 安田女子大学, 薬学部, 教授 (40112156)
• Co-Investigator(Kenkyū-buntansha): INOUE Tsuyoshi 岡山大学, 大学院医歯薬学総合研究科, 准教授 (40370134) ; KATO Hisato 就実大学, 薬学部, 助手 (70639228)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: センサー / アセチルコリン / 海馬 / 覚醒マウス / 光増感剤 / 光不活性化 / シクロデキストリン / ライソシン

Outline of Final Research Achievements

We have developed a miniaturized acetylcholine sensor with high sensitivity and high selectivity and applied it to monitor changes in the concentration of acetylcholine in the hippocampus of awake mice in situ. Also, we have applied electrochemical sensors, such as a K+ sensor and an oxygen electrode, to analyze how porphyrin and chlorin dyes photoinactivate cell membranes. Furthermore, we have applied a K+ sensor to cell membrane permeability assay to clarify the action mechanism of antimicrobial cyclodextrin derivatives with low hemolytic activity and a new antibiotic, lysocin, that targets menaquinone in the bacterial membrane.

Free Research Field

物理系薬学