2015 Fiscal Year Final Research Report
Structural study of replication-coupled DNA repair
| Project/Area Number |
25460038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 構造生物学 |
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| Outline of Final Research Achievements |
In this research, we have carried out structural study of a DNA glycosylase which repairs damaged DNA coupled with DNA replication. DNA glycosylase was expressed and prepared with high purity for crystallization. Crystals of the DNA glycosylase and DNA complex were obtained after the crystallization screening and modification of the conditions. X-ray diffraction experiments were carried out on beam lines at SPring-8 and Photon factory, and the crystal structure was determined. In addition, we measured the glycosylase activity of the wild type and its mutants, and revealed the structural insight into the DNA repair mechanism of the DNA glycosylase.
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| Free Research Field |
医歯薬学
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