Development of novel preparation method of molecularly imprinted polymer for bio-related compounds and its application

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Mukogawa Women's University
• Principal Investigator: HAGINAKA Jun 武庫川女子大学, 薬学部, 教授 (20164759)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分子認識 / 分析科学 / 薬学 / 分子インプリントポリマー

Outline of Final Research Achievements

Molecularly imprinted polymers (MIPs) for chlorogenic acid (CGA) were prepared by modified precipitation polymerization using methacrylic acid as a functional monomer, divinylbenzene as a crosslinker and methanol as a co-solvent. The prepared MIPs were microspheres with a narrow particle size distribution. Binding experiments and Scatchard analyses revealed that two classes of binding sites, high and low affinity sites, were formed on the MIP. In addition to shape recognition, hydrophilic interactions seem to work for the recognition of CGA on the MIP. The MIP had a specific molecular-recognition ability for CGA, while other related compounds, such as caffeic acid, gallic acid, protocatechuic acid and vanillic acid, could not be recognized by the MIP. Furthermore, the MIP for CGA was successfully applied for extraction of CGA in the leaves of Eucommia ulmodies.

Free Research Field

分析科学