2016 Fiscal Year Final Research Report
Functional roles of SV40 microRNA in host antiviral immunity.
| Project/Area Number |
25460075
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Musashino University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI TETSUYUKI 武蔵野大学, 薬学研究所, 講師 (00403692)
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Keywords | マイクロRNA / SV40 / 炎症性サイトカイン / DNA複製 / miR-S1 |
|---|
| Outline of Final Research Achievements |
We explored functional roles of miR-S1 encoded by SV40 virus in DNA replication and cytokine expression within HEK293 cells transfected with SV40 genome vectors. Semi-quantitative qPCR analysis showed that miR-S1-3p rather than miR-S1-5p was more abundantly expressed in cells transfected with SV40 genome vectors. Consistently, reporter assays showed that miR-S1-3p had much more repressive effects on luciferase activity than miR-S1-5p did. Replication rate of viral DNA were evaluated by qPCR analysis in various types of cells transfected with SV40 vectors with or without miR-S1 expression. These results indicated that the replication rate increased proportionally to T antigen expression, but was retarded by its excess expression, often observed in transfected cells without miR-S1 expression. Examination of cytokine expressions in HEK293 cells transfected with SV40 vectors revealed that miR-S1 affected the expression of TNFalpha and IL17F through its modulation of T antigen expression.
|
| Free Research Field |
細胞生物学、分子生物学
|
