Research of the regulatory mechanism of CERT-VAP interaction, from the causing factors to the caused effects.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KUMAGAI KEIGO 国立感染症研究所, 細胞化学部, 主任研究官 (40443105)
• Co-Investigator(Renkei-kenkyūsha): HANADA KENTARO 国立感染症研究所, 細胞化学部, 部長 (30192701)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 脂質輸送 / セラミド / スフィンゴミエリン / FFATモチーフ / リン酸化 / メンブレンコンタクトサイト / クラミジア

Outline of Final Research Achievements

In this research, I tried to identify a kinase that changes the phosphorylation state of serine 315 (S315) of ceramide transport protein (CERT) when its expression level is suppressed by transfection of siRNA library. As a result, I showed that suppressed expression of a certain stress-responsive kinase decreased the phosphorylation state of CERT S315. From the information about the kinase, I speculated that an environmental factor might be involved in phospho-regulation of CERT and experimentally showed that the factor critically up-regulated the phosphorylation state of CERT S315.

Free Research Field

医歯薬学