Application of D-cycloserine (an anti-tubercular agent) biosynthetic genes to drug production

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Hiroshima University
• Principal Investigator: Kumagai Takanori 広島大学, 医歯薬保健学研究院(薬), 准教授 (70274058)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 抗結核薬 / D-サイクロセリン / 遺伝子破壊 / 代謝工学 / 異種生産

Outline of Final Research Achievements

In the present study, high level heterologous production system of an anti-tubercular agent, D-cycloserine (D-CS), by Escherichia (E.) coli was established through co-expression of the four D-CS biosynthetic genes (dcsC, dcsD, dcsE and dcsG) and a self-resistance gene, dcsJ, in combination with metabolic engineering of the E. coli host. In addition, enzymatic functions of DcsA and DcsG involved in the D-CS biosynthesis were clarified by using recombinant proteins expressed in E. coli and purified.

Free Research Field

微生物薬品学