Glycosylation of functional natural compounds for improving intestinal absorption

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: The Makino Memorial Foundation of Kochi Prefecture (2014-2015); Nagoya City University (2013)
• Principal Investigator: Mizukami Hajime 公益財団法人高知県牧野記念財団, その他部局等, その他 (30128219)
• Co-Investigator(Kenkyū-buntansha): TERASAKA Kazuyosi 名古屋市立大学, 大学院薬学研究科, 講師 (60405214)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 生理活性天然物 / 糖鎖構築 / 糖転移酵素 / 消化管吸収

Outline of Final Research Achievements

We have isolate and characterized O- and C-glucosyltransferases from various plants including Gardenia jasminoides, Fragaria ×ananassa and Glycine max. By usig the recombinant glycosyltransferases so far obtained, a wide array of natural glycosides can be easily prepared.
To analyze the relationship between sugar chain structures of natural glycosides and intestinal absorption of these compounds, we have tried to prepare recombinant lactose-phlorizin hydrolase (LPH) originated from human or rat intestine.

Free Research Field

薬用植物学、生薬学