The screening of crude dergs to protect butyrate-induced death of colonic epithelial cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Iwaki Meisei University
• Principal Investigator: Matsumoto Tsukasa いわき明星大学, 薬学部, 教授 (00173906)
• Co-Investigator(Renkei-kenkyūsha): TABUCHI Yoshiaki 富山大学, 研究推進機構, 教授 (20322109) ; YAMADA Haruki 東京薬科大学, 薬学部, 教授 (60096691) ; KAWAGUTI Kiichirou いわき明星大学, 薬学部, 教授 (10146334)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 潰瘍性大腸炎 / 酪酸 / 結腸上皮細胞

Outline of Final Research Achievements

Recent findings demonstrated that a significant number of Fusobacterium varium was identified in the mucosa of patients with ulcerative colitis (UC), and it has been suggested that the butyrate produced by F. varium involved in the pathogenesis of UC. In the screening of Sino-Japanese herbal medicines to protect butyrate-induced death of murine colonic epithelial cells MCE301 as an in vitro model of UC, it was found that the extract of Alisma orientale Juzepczuk (Alismataceae) showed potent protective activity against butyrate-induced cell death. Bioassay-guided purification of methanol extract of the rhizome of A. orientale let to the isolation of alosol B and alisol B acetate. These results suggest that the use of alosol B and alisol B acetate, or a crude extract of A. orientale containing these compounds are expected the effectiveness to relieve UC caused by the butyrate-induced death of colonic epithelial cells.

Free Research Field

生薬学