2015 Fiscal Year Final Research Report
Production of new triterpene and establishment of high production system of triterpene
| Project/Area Number |
25460142
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | トリテルペン / トリテルペン合成酵素 / ゲノム遺伝子 / シロイヌナズナ / ダイズ / ルペオール |
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| Outline of Final Research Achievements |
Eleven triterpene synthase homologs are found in Glycine max genome. Among them, one homolog (locus name: Glyma.20G192700) is not transcribed in G. max. This gene was amplified by PCR with genome DNA as a template, from which introns were removed. Resultant DNA was expressed in Saccharomyces cerevisiae. Accumulated product in yeast cells was analyzed by GC-MS, and identified as lupeol which is known compound. Although this result is deviated substantially from original purpose to produce new triterpene, it is succeeded in revealing that lupeol had occurred, or will occur in G. max, by functional analysis of the genomic gene.
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| Free Research Field |
天然資源系薬学
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