Molecular mechanism and regulation of immune activation cascade by aminopeptidase contained exosome.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460172
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Teikyo Heisei University
• Principal Investigator: OGAWA Yuko 帝京平成大学, 薬学部, 准教授 (30267330)
• Co-Investigator(Renkei-kenkyūsha): TSUJIMOTO Masafumi 帝京平成大学, 薬学部, 教授 (00281668) ; YANOSHITA Ryohei 帝京平成大学, 薬学部, 教授 (90267336) ; GOTO Yoshikuni 帝京平成大学, 薬学部, 講師 (90455345)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: exosome / マクロファージ / DPP IV / 唾液 / LPS / Toll like receptor 4 / 一酸化窒素 / 細胞間情報伝達

Outline of Final Research Achievements

Human whole saliva contains two types of extracellular vesicles. One type highly expresses dipeptidyl peptidase-4 (DPP4) and the other type rarely expresses them. We designate former as DPP IV-exosome (DPP4exo), and later as deficient DPP IV-exosome (dDPP4exo). DPP4exo contained lipopolysaccharide (LPS) abundantly, and dDPP4exo contained it slightly. Although large amount of LPS interacted weakly with DPP4exo or existed separately forming micelle, part of LPS bind DPP4exo tightly. DPP4exo alone did not activate murine macrophage, however, DPP4exo with interferon-gamma produce high level of nitric oxide (NO) from murine macrophage. dDPP4exo did not cause NO production, even when interferon-gamma was added. DPP4exo is presumed to induce NO production from macrophage, when inflammation caused by bacterial infection, and associated with increased levels of interferon-gamma in oral cavity.

Free Research Field

衛生化学