2015 Fiscal Year Final Research Report
Mechanisms of prostanoids release from the cells
| Project/Area Number |
25460184
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
|
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| Research Institution | Tohoku University (2014-2015)
Hokkaido University (2013) |
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Principal Investigator |
|
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | プロスタノイド / トランスポーター / 細胞外放出 |
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| Outline of Final Research Achievements |
The purpose of this study is to estimate the contribution of transporters to the prostanoids release from the cells. We established the LC/MS/MS analytical methods and applied to the in vitro studies using determination of prostanoids released from culture cells and vesicullar transport assay. In the experimentes using MRP inhibitors and MRP4 siRNA, MRP4 partly contributes to the release of PGE2 and PGF2α but not TXB2 from A549 cells. EPA-derived prostanoids also showed similar results.
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| Free Research Field |
薬物動態学
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