2016 Fiscal Year Final Research Report
Development of a model for the monitoring ontogenic expression of CYP3A by used of secreted reporter-expressing iPS cells
| Project/Area Number |
25460195
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Medical pharmacy
|
|---|
| Research Institution | University of Shizuoka (2015-2016)
Tohoku Pharmaceutical University (2013-2014) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Keywords | iPS細胞 / CYP3A4 / レポーター遺伝子 / 肝細胞 |
|---|
| Outline of Final Research Achievements |
Human induced pluripotent stem cells (iPSCs) are a valuable source of hepatocytes for applications in drug development studies. Human CYP3A genes show unique expression changes, which are influenced by human development, and this developmental switch in expression between CYP3A4 and CYP3A7 genes occurs during the first 1 - 2 years after birth. In this study, based on the reproduction of the CYP3A expression pattern in the development, we aimed to establish methods for the differentiation of iPSCs into functional hepatocytes and evaluate novel transcriptional mechanism of pharmacokinetic-related genes. As results, we identified novel mechanisms involved in the induction of CYP3A4 and MRP3, and modified our previously established methods based on these results.
|
| Free Research Field |
薬物代謝
|
