2015 Fiscal Year Final Research Report
Reconstruction of vaginal microbiota to prevent and treat miscarriage and premature birth
| Project/Area Number |
25460226
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Meiji Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 膣内細菌叢 / 妊婦 / Lactobacillus |
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| Outline of Final Research Achievements |
Miscarriage and premature birth occur due to an unbalance in the vaginal microbiota. Therefore, novel prevention and therapeutic method should be ble to reconstruct the unbalance in vaginal microbiota. During the last three years, we elucidated the following three issues; 1) Predominant Lactobacillus sp. 1 in pregnant women was identified by comprehensively analyzing vaginal microbiota using pyrosequencing method. 2) Cytotoxicity due to Gardnerella sp. was reduced in the presence of Lactobacillus sp. 1 on HeLa cell and also Lactobacillus sp. 1 inhibited the production of inflammatory cytokine. 3) LAMP (Loop-Mediated Isothermal Amplification) method was developed in order to detect Gardnerella sp. with high sensitivity and rapidly.
These findings suggested that Lactobacillus sp. 1 has become a candidate of probiotics to prevent or treat bacterial vaginosis.
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| Free Research Field |
医歯薬学
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