DISC1 regulates NRG1/ErbB signal pathway

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460284
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Nagoya University
• Principal Investigator: MORI DAISUKE 名古屋大学, 脳とこころの研究センター, 特任准教授 (00381997)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 統合失調症 / DISC1 / Neuregulin / 細胞内輸送

Outline of Final Research Achievements

DISC1 is a candidate gene for susceptibility to schizophrenia. We have reported that DISC1 protein is accumulated on Golgi apparatus in cultured neurons, which suggests that it is a component of transporting vesicles budding from Trans-Golgi network. However, the functional significance of DISC1 on Golgi-apparatus has been essentially unknown. Here, we report that DISC1 interacts with intracellular domain of precursor pro-Neuregulin-1 (pro-NRG1) and regulates its secretion and then propose our hypothesis that DISC1 promotes the formation and budding of vesicles including DISC1-interacting proteins. For the more strict quantification of secretion, we developed a synchronized budding system of single-passed transmembrane protein through Golgi apparatus, and revealed that the budding of pro-NRG1 and the secretion of mature NRG1 were relatively impaired in full-length DISC1 deficient hippocampal neuron.

Free Research Field

分子生物学