2016 Fiscal Year Final Research Report
Elucidation of mechanism of real-time molecular dynamics of second messengers using caged compounds
Project/Area Number |
25460288
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General physiology
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Keywords | パッチクランプ法 / 嗅細胞 / 線毛 / ケージド化合物 / 細胞内因子 |
Outline of Final Research Achievements |
Sense of olfaction starts at the olfactory cilia of the olfactory receptor cells. Two types of olfactory transduction channels expresses on the cilia with high density, named CNG and Cl(Ca) channels. These channels cause current responses, which leads to the signals transmission to the brain. Spatial and temporal distributions of cytoplasmic elements (cAMP, Ca2+) are key factors in this cascade. However, structure of cilia are extremely tiny (φ100nm). This is the one of the reason for delaying electrophysiological study of the olfactory receptor cell. Furthermore, we combined three techniques into one set up to examine the excitatory and inhibitory of transduction channels and dynamics of second messengers in single cilium. Three techniques are here. 1) LMS-ROI. 2) Caged substances and photolysis, 3) Patch-clamp recording in parallel. Therefore, we observed the channel inhibition by certain odorants and molecular kinetics in the cilium in the real time using the patch clamp recording.
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Free Research Field |
生理学、電気生理学、神経科学
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