Identification of bone formation mechanisms at resorption lacunae

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Keio University
• Principal Investigator: Kuroda Yukiko 慶應義塾大学, 医学部, 助教 (70455343)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨形成 / 糖鎖修飾 / 骨吸収窩 / 破骨細胞 / 骨芽細胞 / 糖タンパク質

Outline of Final Research Achievements

Abundant glycoproteins exist in bone resorption pits, whereas their physiological roles remain unknown. In this study, I observed that glycoproteins sticking to the surface of resorption pits were produced by mature osteoclasts, but not osteoclast precursors. I also found a novel lectin that tightly bound to a bone reporption pit by lectin microarray analysis, and this lectin specifically recognized osteoclasts and blood vessels in vivo. Matrix metalloproteinase was identified as a major protein that bound to the lectin in those mature osteoclast secreted. Finally, the osteoblast culture experiment suggested the possibility that bone resorption pits could modulate osteoblast migration.

Free Research Field

骨生物学