2015 Fiscal Year Final Research Report
Biochemical analysis of clathrin heavy chain, CHC17 and CHC22
| Project/Area Number |
25460338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | クラスリン / 細胞内小胞輸送 / 核移行 |
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| Outline of Final Research Achievements |
Clathrin heavy chains (CHCs) are known as essential molecules for intracellular vesicular transport. Recently, some reports revealed that these play important roles in mitosis and transfer. In this study, we showed that inhibition of mitochondrial respiration increased CHC17, one of CHC isoforms, in nuclear fraction by using biochemical analysis. However, immunohistorogical analysis did not show this phenomenon. Through these experiments, we revealed the biochemical characteristics of CHC22, another isoform of CHC. By using this, we success to separate CHC17 and CHC22.
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| Free Research Field |
薬理学
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