2015 Fiscal Year Final Research Report
Physiological function of a novel HPD1 highly expressed in fatty liver
| Project/Area Number |
25460348
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TAKIGUCHI SOICHI 独立行政法人国立病院機構, 九州がんセンター臨床研究センター, 室長 (00280793)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 脂肪細胞 / 脂肪肝 / 核内受容体 |
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| Outline of Final Research Achievements |
Hepatic peroxisome proliferator-activated receptor γ (PPARγ)-dependent gene 1 (HPD1) gene was isolated as high expression in fatty liver and unknown function. The HPD1 gene is directly regulated by PPARγ. In the present study, to assess physiological function of HPD1 protein, HPD1 expression was repressed by lentivirus expressing HPD1shRNA. Infection of lentiHPD1shRNA dramatically decreased HPD1 protein by about 20% as comparison with lenticontrolshRNA. Although normal or phospho-specific antibody array was performed using lenticontrol and HPD1shRNA-infected cells,there was no significant difference between the protein or phosphorylation levels of both cells. To assess subcellular localization of HPD1 protein, 3T3-L1 adipocytes were fractionated by differential centrifugation. The result showed that the HPD1 is exclusively detected in the microsomal fraction. Interestingly, HPD1 localized in detergent-insoluble microsomal fraction.
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| Free Research Field |
分子生物学
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