2015 Fiscal Year Final Research Report
A novel RNA metabolism involved in cell differentiation and survival
| Project/Area Number |
25460371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kochi University |
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Principal Investigator |
Shuji Sakamoto 高知大学, 教育研究部医療学系基礎医学部門, 准教授 (80397546)
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| Research Collaborator |
Todaka Hiroshi 高知大学, 教育研究部医療学系基礎医学部門, 助教 (80769662)
Higuchi Takuma 高知大学, 教育研究部医療学系基礎医学部門, 助教 (10754567)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 二本鎖RNA結合蛋白質 / microRNA生合成 / miRNA / 筋分化 / 中心核病 / Dynamin 2 / NF90 / NF45 |
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| Outline of Final Research Achievements |
1. The nuclear factor 90 (NF90) or the complex of NF90 with NF45 is known to be involved in microRNA biogenesis, mRNA stabilization, translational repression, replication of viral RNA and transcription in vitro. In this study, we found that the NF90-NF45 complex induces centronuclear myopathy through increased expression of dynamin 2, a causative gene of the disease, by an NF90-NF45-induced reduction of miR-133a expression in vivo.
2. We observed that the NF90-overexpressing HEK293 cells did not exhibit longer-term survival than the control cells transfected with mock plasmids when glucose was withdrawn, suggesting that cellular energy balance was not influenced by overexpression of NF90 in cultured cells in vitro.
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| Free Research Field |
分子生物学
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