2015 Fiscal Year Final Research Report
Search for the intracellular substrates and activators of coagulation factor XIII (plasma transglutaminase)
| Project/Area Number |
25460379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 酵素 / トランスグルタミナーゼ / 凝固第XIII因子 / 細胞内基質 / 細胞内活性化 |
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| Outline of Final Research Achievements |
The A subunit of coagulation factor XIII (FXIII-A) is a plasma transglutanimase (TGase), which exists not only in plasma but also inside bone marrow-derived cells such as megakaryocytes and monocytes/macrophages. In the present study, we examined the intracellular activation and activity of FXIII-A. We found that there was an activation factor(s) of FXIII-A in nucleus in the absence of proteolytic cleavage of the activation peptide in FXIII-A, and that at least three nuclear proteins were identified as possible substrates for FXIII-A. Furthermore, our study suggested that amine-modification of proteins by TGase activity regulates functions of the substrate proteins by the acceleration of proteasomal degradation in the absence of ubiquitination.
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| Free Research Field |
医歯薬学
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