2015 Fiscal Year Final Research Report
An integrated histopathological and cytogenetic analysis of intratumor heterogeneity of liposarcoma and its diagnostic application
Project/Area Number |
25460448
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
|
Research Institution | Tokyo Metropolitan Komagome Hospital (Clinical research laboratory) |
Principal Investigator |
Motoi Toru 東京都立駒込病院(臨床研究室), その他部局等, その他 (50291315)
|
Co-Investigator(Kenkyū-buntansha) |
KATO Ikuma 東京都立駒込病院, 病理科, 医員 (80644939)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | 脂肪肉腫 / 腫瘍内多様性 / 遺伝子増幅 / CISH法 / MDM2 |
Outline of Final Research Achievements |
Well-differentiated liposarcoma(WLPS) and dedifferentiated liposarcoma(DLPS) are characterized by MDM2 gene amplification and resultant protein overexpression. In this study we examined cytogenetic, mRNA and protein status of MDM2 using a novel approach of simultaneous visualization of morphology, cytogenetic and molecular abnormalities. Although MDM2 abnormalities were only observed in a subset of tumor cells, clonal selection and condensation of these abnormal cells was occurred during dedifferentiation process, concurrently with loss of adipocytic differentiation. Present study may provide a new information useful for pathological diagnosis of liposarcomas as well as future molecular targeted therapy using MDM2 inhibitors.
|
Free Research Field |
人体病理学
|