An integrated histopathological and cytogenetic analysis of intratumor heterogeneity of liposarcoma and its diagnostic application

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460448
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Tokyo Metropolitan Komagome Hospital (Clinical research laboratory)
• Principal Investigator: Motoi Toru 東京都立駒込病院(臨床研究室), その他部局等, その他 (50291315)
• Co-Investigator(Kenkyū-buntansha): KATO Ikuma 東京都立駒込病院, 病理科, 医員 (80644939)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 脂肪肉腫 / 腫瘍内多様性 / 遺伝子増幅 / CISH法 / MDM2

Outline of Final Research Achievements

Well-differentiated liposarcoma(WLPS) and dedifferentiated liposarcoma(DLPS) are　characterized by MDM2 gene amplification and resultant protein overexpression. In this study we examined cytogenetic, mRNA and protein status of MDM2 using a novel approach of simultaneous visualization of morphology, cytogenetic and molecular abnormalities. Although MDM2 abnormalities were only observed in a subset of tumor cells, clonal selection and condensation of these abnormal cells was occurred during dedifferentiation process, concurrently with loss of adipocytic differentiation. Present study may provide a new information useful for pathological diagnosis of liposarcomas as well as future molecular targeted therapy using MDM2 inhibitors.

Free Research Field

人体病理学