Semi-comorehensive analysis of gene amplification in solid carcinomas using MLPA and FISH

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460452
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Kanazawa University
• Principal Investigator: Ooi Akishi 金沢大学, 医学系, 教授 (50160411)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 遺伝子増幅 / MLPA / FISH / 分子標的療法 / 胃癌 / 乳癌 / 大腸癌 / 脂肪肉腫

Outline of Final Research Achievements

Gene amplification is a major type of genetic aberration found in human cancers of solid types.　The amplified genes so far have been useful targets or will be potential targets for molecular therapies. In this study, cancers of the stomach, breast and colon, which are frequent among Japanese, were examined for amplification of 46 genes by multiplex ligatioin-dependent probe amplification followed by fluoresence in situ hybridization. The results showed that the genes coding receptor tyrosine kinases (ERBB2, FGFR1, FGFR2, EGFR, MET), regulatory genes of cell cycle progression (CCNE1, CCND1, CDK6, MDM2), and gene coding a transcription factor (MYC) were found frequently amplified in these tumors.
In conclusion, combination of MLPA and FISH analysis is a feasible approach for obtaining the semi-comprehensive genetic information that is necessary for personalized molecular targeted therapy.

Free Research Field

人体病理学