DNA methylation alterations in autoimmune pancreatitis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Shinshu University
• Principal Investigator: UEHARA Takeshi 信州大学, 学術研究院医学系, 准教授 (80402121)
• Co-Investigator(Kenkyū-buntansha): MASUMOTO Junya 愛媛大学, 大学院学医学系研究科ゲノム病理学分野, 教授 (20334914)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 自己免疫性膵炎 / メチル化解析

Outline of Final Research Achievements

We used an Illumina Infinium MethylationEPIC Kit to conduct a comprehensive analysis of DNA methylation in tissues of patients with autoimmune pancreatitis (AIP). Controls included pancreatic tissue acquired from patients with obstructive pancreatitis caused by pancreatic carcinomas as well as from normal pancreatic tissue. We compared genes with promoter hypermethylation between patients with AIP and the controls. The validation of the method was affected by technical problems. Immunohistochemical analysis will be required to determine if promoter hypermethylation of specific genes is involved in the pathogenesis of AIP.

Free Research Field

IgG4関連疾患