2016 Fiscal Year Final Research Report
Molecular and functional analysis of YAP/TAZ protein complex
| Project/Area Number |
25460482
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Aichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | がん遺伝子 / 転座 |
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| Outline of Final Research Achievements |
The TAZ/CAMTA1 or YAP/TFE3 fusion genes have been identified in epithelioid hemangioendothelioma (EHE). However, the detailed function of TAZ/CAMTA1 and YAP/TFE3 on cell growth or motility remains unclear. To investigate the biological roles of these fusion proteins in EHE, we generated lentivirus mediated TAZ/CAMTA1 or YAP/TFE3 expression system in HMEC1 cells, and analyzed the changes in cell growth, motility, and the interacting proteins with fusion protein using a tandem affinity purification with LC-MS/MS. Fusion proteins were localized in the nucleus, whereas TAZ and YAP proteins preferentially found in the cytoplasm. Expression of TAZ/CAMTA modestly increased cell proliferation and motility. Using tandem affinity purification coupled with LC-MS/MS, we identified several TAZ/CAMTA-associated proteins, such as ERG, SIR7, AHNK. Our results will provide new insights into development of EHE, and give some clues to developing a new molecular target therapy for EHE.
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| Free Research Field |
腫瘍
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