Investigation of c-ktlow murine hematopoietic stem cells for the role in aged animals

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460483
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kansai Medical University
• Principal Investigator: SASAKI Yutaka 関西医科大学, 医学部, 准教授 (80425066)
• Co-Investigator(Renkei-kenkyūsha): SONODA Yoshiaki 関西医科大学, 医学部, 教授 (60206688)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 造血幹細胞 / G0 / c-kit

Outline of Final Research Achievements

Hematopoietic stem cells (HSCs) stay long in G0 of cell cycle status. The c-kitlow subfraction of the Lin-Sca-1+c-kit+CD150+CD48-HSCs contains cells in G0 in high frequency. Observation in vivo of integrated BrdU in HSCs suggested that HSCs divided slowly with fluctuating c-kit expression in the time course. The c-kitlow subfraction included cells that did not divide in response to pro-proliferation stimuli. These cells were poor in mitochondrial mass and in activities of reactive oxygen species. DNA microarray analyses indicated that c-kitlow cells differed from c-kithigh cells in the level of cell cycle related gene expression as well as genes for transcription, phosphorylation and metabolism.

Free Research Field

造血幹細胞