Functional in vivo cellular imaging of graft-versus-host disease in allogeneic hematopoietic stem cell transplantation in mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460509
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: National Cancer Center Japan
• Principal Investigator: Ikarashi Yoshinori 国立研究開発法人国立がん研究センター, 研究所, 外来研究員 (70280782)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: イメージング / 同種造血幹細胞移植 / 移植片対宿主病 / GFP

Outline of Final Research Achievements

Visualizing the function of donor cells after allogeneic hematopoietic stem cell transplantation (HSCT) will enable understanding of the process of graft-versus-host disease (GVHD). In this study, we visualized the proliferation and apoptosis of green fluorescent protein protein (GFP) donor cells using immunofluorescence staining and in vivo fluorescence imaging. Immunofluorescence staining revealed that a large fraction of donor cells were cell proliferation marker, Ki-67 positive and a few host but not donor mononuclear cells were apoptotic cell marker, cleaved Caspase-3 positive in the secondary lymphoid organ at early phase after HSCT. We could not visualize apoptotic cell using in vivo fluorescence imaging. However, it is very important for visualizing cellular proliferation and apoptosis in vivo to understand the process of GVHD.

Free Research Field

免疫学