Screening of the RBC receptor molecules for malaria merozoite invasion based on wheat germ cell free system

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460517
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Ehime University
• Principal Investigator: Takashima Eizo 愛媛大学, プロテオサイエンスセンター, 准教授 (50366762)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: マラリア / 赤血球

Outline of Final Research Achievements

Merozoite invasion of malaria parasite is attributed with the parasite erythrocyte binding antigens (EBA). In this study, we attempted to screen for identification of the receptor molecules on the surface of the host RBC for the EBAs. By means of comprehensive immuno-precipitation with more than 200 polyclonal antibodies, we identified the molecule interacting with GPI-anchored micronemal antigen (GAMA), a blood stage vaccine candidate. We named the molecule GAMA association protein (GAAP). Biacore analysis revealed that Kd value of the interaction was 2.5 nM. We also identified the GAAP interacting domain in GAMA which has about 100 amino acid residues.

Free Research Field

寄生虫学