Proteomic analyses of mitochondrial respiratory chains from parasitic helminths inhabiting various tissue environments

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460519
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Juntendo University
• Principal Investigator: Takamiya Shinzaburo 順天堂大学, 医学部, 非常勤講師 (90138206)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 寄生蠕虫 / 低酸素適応 / 呼吸鎖 / シトクロムｂ５ / ミトコンドリア / プロテオーム

Outline of Final Research Achievements

Using parasitic helminths inhabiting various tissue environments, this research project was undertaken to elucidate underlying mechanisms for their infectious establishment and maintenance by analyzing expression and regulation of mitochondrial proteins, especially respiratory chains, responding to changing oxygen tension. Comparative mass spectrometric analyses, using 2D-PAGE followed by in-gel digestion and shotgun method, of mitochondrial proteins of Ascaris suum adult and Caenorhabditis elegans dwelling in anaerobic host intestine and free-living under aerobic conditions, respectively, revealed that the protein profiles were characteristic of the respective nematodes. The larva of Spirometra erinaceieuropaei living under dermal tissues, like Paragonimus westermani, was shown to possess both aerobic and anaerobic mitochondria.

Free Research Field

生化学、分子細胞生物学