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2015 Fiscal Year Final Research Report

Elucidation of a synthetic pathway for complex-type N-linked glycans in Trypanosoma brucei

Research Project

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Project/Area Number 25460522
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionMatsuyama University

Principal Investigator

Nakanishi Masayuki  松山大学, 薬学部, 准教授 (00281048)

Project Period (FY) 2013-04-01 – 2016-03-31
Keywordsトリパノソーマ原虫 / 糖鎖 / 糖タンパク質
Outline of Final Research Achievements

Trypanosoma brucei, a causative parasite of African Sleeping sickness, is thought to require the expression of complex-type N-linked glycans for its proliferation. However, little is known about the responsible enzymes for the glycan synthesis. Especially, lack of a homolog to GnT-I was a mystery for years. This project focused on the identification of the enzyme of T. brucei (TbGnT-I). Although TbGT11 was reported as the TbGnT-I in the middle of this project, it was still not clear whether the enzyme was alone sufficient to catalyze GnT-I activity. We found that native TbGnT-I showed a size of ca. 400 kDa by a gel filtration chromatography, and overexpressed TbGT11HA3 was also contained in the fraction. The recombinant TbGT11HA3 showed no activity when expressed in other than T. brucei. An N-linked glycosylation on TbGT11 was essential for the activity. These results strongly suggested that TbGnT-I was a 400-kDa protein complex containing glycosylated TbGT11 as an essential component.

Free Research Field

生化学・分子寄生虫学

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Published: 2017-05-10  

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