2016 Fiscal Year Final Research Report
Study on the mechanisms involved in insecticide resistance of vector mosquiotes by genome editing technology
| Project/Area Number |
25460525
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Tomita Takashi 国立感染症研究所, 昆虫医科学部, 室長 (20180169)
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| Research Collaborator |
ITOKAWA Kentaro 国立感染症研究所, 昆虫医科学部, 流動研究員 (70769992)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 殺虫剤抵抗性 / シトクロムP450 / 遺伝子重複 / 遺伝子過剰発現 / シス作用性変異 / 遺伝子ノックアウト / ネッタイイエカ |
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| Outline of Final Research Achievements |
Genetic studies have revealed that pyrethroid resistance of Culex quinquefasciatus is strongly associated with overexpressing CYP9M10 haplotypes. In this study, we demonstrated that a point mutation in the core promotor region of CYP9M10 is attributable to the elevated transcription. Moreover, bioassays using CYP9M10-knocked out mosquitoes derived from a pyrethroid resistant strain proved the primary responsibility of CYP9M10 for metabolic resistance to permethrin in larval stages.
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| Free Research Field |
昆虫毒物学，衛生動物学，遺伝学
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