Elucidation of the mechanism of Cholix cytotoxicity via its receptor.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460526
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Chiba University
• Principal Investigator: Yahiro Kinnosuke 千葉大学, 医学(系)研究科(研究院), 准教授 (80345024)
• Co-Investigator(Renkei-kenkyūsha): OGURA Kohei 国立国際医療センター研究所, 博士研究員 (00586612)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 毒素 / サイトカイン / 受容体 / 細胞死

Outline of Final Research Achievements

We investigated the cytotoxic effect of Cholix on HepG2 cells in the presence or absence of an inflammatory cytokine. Cytotoxicity of Cholix was dramatically enhanced with a cytokine in HepG2 cells. The mechanism of Cholix-induced cell death was involved in the signal transduction of MAPK, PKC or AMPK. In addition, we made a new peptide antibody for Cholix to identify the receptors on cell surface by immunoprecipitation. After biotin-labeled cell lysates were incubated with Cholix in the presence of anti-cholix peptide antibody, Cholix interaction proteins were detected by Western blotting using avidin-HRP. We observed two bands, which proteins were analyzed by TOF-Ms/Ms analysis.However, we could not identify these proteins. Now, we collect these bands to analyze again.

Free Research Field

細菌学