Functional analysis of Vibrio parahaemolyticus T3SS2

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460531
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka University
• Principal Investigator: KODAMA TOSHIO 大阪大学, 微生物病研究所, 准教授 (20346133)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 腸炎ビブリオ

Outline of Final Research Achievements

In this study, I aimed to determine a pathogenic mechanism of food-borne pathogen, Vibrio parahaemolyticus.
I found that V. parahaemolyticus has two distinct mechanisms of diarrhea, T3SS2-effector (VopV) and T3SS2-toxin (TDH), assessed by rabbit ileal loop model. Hemolytic activity of TDH was necessary for T3SS2-TDH mediated enterotoxicity. All T3SS2 effectors that have already identified did not involve in this enterotoxicity. Therefore, we tried to identify new effector by proteomic approach and have gotten one candidate protein. The involvement of this effector candidate protein in T3SS2-TDH mediated enterotoxicity is currently under investigation.

Free Research Field

細菌学