2015 Fiscal Year Final Research Report
Roles of retron and msDNA in regulation of virulence expression and genome evolution
| Project/Area Number |
25460532
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bacteriology (including mycology)
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIMAMOTO TOSHI 広島大学, 大学院生物圏科学研究科, 助教 (70583136)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 病原性 / レトロン / コレラ菌 / msDNA / ビブリオ |
|---|
| Outline of Final Research Achievements |
Relationship between pathogenicity and retron-Vc95 in Vibrio choelare O1/O139 serotypes has been investigated in this study. Candidate proteins that can interact with ORF540 or ORF205, encoded by the genes located downstream of the ret gene, of unknown functions were searched. One of the candidates, ArcB that regulates genes related to anaerobic respiration, was found. The arcB-defective mutant was found to have lower pathogenicity to silk warm larvae than the wild type. VC0176 proten, one of the candidate proteins that can bind to msDNA-Vc95, was purified. Binding of the VC0176 protein to the promoter region of VC0177 gene and msDNA-Vc95 was confirmed.
|
| Free Research Field |
食品衛生学，分子細菌学
|
